Figure 7.
A humoral factor present in the serum of EC-Phd2Hif2 mice is sufficient to suppress proinflammatory responses in ECs. (A) Experimental scheme for HPAECs subjected to 0.5% O2 for 18 hours in the presence of 10% mouse serum isolated from EC-Phd2Hif1, EC-Phd2Hif2 or corresponding Cre- mice followed by reoxygenation for 6 hours in the presence of 1 ng/ml TNFα. Cells cultured under normoxic conditions in the presence of 10% mouse serum from mice with the aforementioned genotypes were used as controls. (B) Shown are transcript levels of VCAM1 and IRF1 in HPAECs subjected to each experimental condition (EC-Phd2Hif1 serum, VCAM1 P=0.04, IRF1 P=0.04; EC-Phd2Hif1 serum, VCAM1 P=0.001, IRF1 P=0.0001; one-way ANOVA with Sidak correction for multiple comparisons). Experiments were repeated twice. Error bars represent SEM. *P<0.05, ***P<0.001.