Enarodustat activated HIF and induced erythropoietin production in BTBR ob/ob mice. (A) Hematocrit levels of BTBR ob/ob mice treated with either vehicle only or different doses of enarodustat at 4, 10, 16, and 22 weeks of age. Enarodustat was administered from 4 to 22 weeks of age. (B) Plasma erythropoietin of BTBR ob/ob mice at 22 weeks of age. They were treated with either vehicle only or enarodustat at a concentration of 0.05 mg/g food. (C) HIF-1α staining of kidney. Data are expressed as means±SEM; vehicle only, n=4–6; low (0.015 mg/g), n=8; intermediate (0.05 mg/g), n=7; high (0.15 mg/g), n=8. *P<0.05; ***P<0.001 compared with the vehicle-only group.