Figure 5.

Glomerular CCL2/MCP-1 expression and macrophage infiltration were suppressed in the enarodustat-treated BTBR ob/ob mice. (A) Transcriptome analysis of isolated glomeruli (n=3 for each group). The initial screening identified 8965 probes, whose absolute values of log₂ (BTBR ob/ob mice treated with enarodustat/BTBR ob/ob mice treated with vehicle only) exceeded 0.5. Comparison of their expression levels between BTBR ob/ob mice and wild-type mice revealed 71 genes that were significantly upregulated (red) and 47 that were significantly downregulated (green) in BTBR ob/ob mice compared with wild-type mice. Normalized expression values of Ccl2/Mcp1 are shown as individual value plots. (B) Urinary CCL2/MCP-1 at 22 weeks of age. (C) Representative images of CD68 staining of kidney. The numbers of CD68-positive cells in ten glomeruli were counted for each mouse. No CD68-positive cells were detected in the glomeruli of BTBR wild-type mice. Data are expressed as means±SEM; wild-type, n=3; ob/ob vehicle, n=6; ob/ob enarodustat, n=7. **P<0.01.