Figure 3.

IC MR gene ablation reduces pendrin protein abundance and pendrin's relative abundance in the most apical 10% of ICs. in mice given either an aldosterone infusion alone or aldosterone plus amiloride. (A and B) Shown is a representative pendrin immunoblot (A) of kidney lysates from IC MR null and wild-type mice following a NaCl-replete diet with an aldosterone infusion (treatment 4), with its respective band density (B). (C) Pendrin and MR double-labeling in a CNT and a CCD from a representative cortical section taken from an aldosterone-treated IC MR null and a wild-type littermate. (D) Pendrin label in the most apical 10% relative to label across the entire cell (redistribution ratio) in both CCD and CNT. (E) A representative pendrin immunoblot of kidney lysates from IC MR null and wild-type mice after an aldosterone infusion with a NaCl-replete diet containing amiloride (treatment 5). Pendrin band density in lysates from each group is shown in (F). (G) Pendrin labeling in a representative cortical section from an amiloride and aldosterone-treated IC MR null and a wild-type littermate. (H) Pendrin label in MR(+) (arrows) and MR(−) (arrowheads) ICs from a cortical section of an IC MR null mouse. (I) Pendrin label per cell (left) and pendrin label in the most apical 10% relative to label across the entire cell (redistribution ratio, right) were quantified in both the MR(+) and MR(−) ICs. *P<0.05.