Fig. 2. PAK signaling with group I PAK inhibition.

TPC1, SW1736, K1, and FTC133 cells were treated with increasing doses of G-5555 or FRAX1036 for 24 hours to avoid significant toxicity. Western blots of downstream PAK targets were conducted to confirm drug activity. GAPDH was used as a loading control and is shown below blots on the same membrane. For SW1736 G-5555, pAKT S473 was blotted on the same membrane as the first GAPDH and is grouped accordingly. At least two biological replicates were performed per cell line and results were similar.