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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Endocr Relat Cancer. 2019 Aug;26(8):699–712. doi: 10.1530/ERC-19-0188

Fig. 6. Effects of G-5555 on cell migration and invasion.

Fig. 6.

TPC1 and FTC133 were seeded on top of Boyden chamber inserts without (A) or with (B) Matrigel coating. Cells were treated for 16 hours with 0, 0.25, or 1 μM G-5555. The top and bottom of the inserts were stained with crystal violet. For migration (A), percent migrated was calculated by comparing the OD of extracted crystal violet from the top and the bottom of the insert. For effects on invasion (B), the fraction of the control (i.e. 0 μM G-5555) was calculated after counting the number of purple pixels from images of the bottom layer. (C) Representative images of invading cells are shown. (D) WST-8 viability assays were conducted in parallel and indicate no cytotoxic effects in these conditions. Cells were seeded in duplicate and at least three biological replicates for each cell line were performed. Data are represented as means + SD. ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05.