Table 1. Number of total animals that used in the experimental groups and removed from the study.
| Experimental animals: Male Wistar Rats (250-300g) |
Class | Experiment | Groups | # of animals | Total number |
| Behavioural studies N = 6 |
Open field test | Control | 48 Rats | 172 Rats | |
| I/R | |||||
| Morris water maze test | I/R+DMSO | ||||
| I/R+MK-4 | |||||
| Y maze test | Control | ||||
| I/R | |||||
| I/R+DMSO | |||||
| I/R+MK-4 | |||||
| Histological studies N = 5 |
TUNNEL staining | Control | 40 Rats | ||
| I/R | |||||
| Nissl staining | I/R+DMSO | ||||
| I/R+MK-4 | |||||
| Tissue analysis N = 6 |
Brain water content assay | Control | 24 Rats | ||
| I/R | |||||
| I/R+DMSO | |||||
| I/R+MK-4 | |||||
| Biochemical studies N = 5 |
Nitrate/Nitrite and Superoxide dismutase assays |
Control | 20 Rats | ||
| I/R | |||||
| I/R+DMSO | |||||
| I/R+MK-4 | |||||
| ELISA | Control | 20 Rats | |||
| I/R | |||||
| I/R+DMSO | |||||
| I/R+MK-4 | |||||
| Molecular study N = 5 |
Real-time PCR | Control | 20 Rats | ||
| I/R | |||||
| I/R+DMSO | |||||
| I/R+MK-4 | |||||
| Removed animals | Death during and after surgery | 53 Rats | |||
| Motor activities impairment | |||||
| Visual deficits | |||||