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. 2020 Mar 9;15(3):e0226395. doi: 10.1371/journal.pone.0226395

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© 2020 Kennedy et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Box-and-whisker plot of the size distribution of MCPs analyzed with various techniques. Note that apparent size and distribution varies widely with each technique. A single-factor ANOVA test revealed that populations differed significantly (p < 0.001). The only populations that are not significantly different (as defined by a p-value greater than .001 in a two-tailed t-test) are TEM of purified MCPs vs thin section TEM and SEM of purified MCPs vs TEM of dehydrated samples (p = .12 and .26, respectively). N = 300 for all, where 100 measurements were made for each of three biological replicates (three different MCP growths and purifications). Abbreviations: transmission electron microscopy (TEM), transmission electron microscopy with samples dehydrated in hexamethyldisilazane (TEM + HMDS), scanning electron microscopy (SEM), cryo transmission electron microscopy (Cryo TEM), ultra-thin section transmission electron microscopy (UTS TEM).