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. 2020 Mar 5;14:993–1001. doi: 10.2147/DDDT.S231814

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© 2020 Qiu et al.

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BDMC inhibited the activation of the Akt signaling pathway and proliferation of hepatocellular carcinoma cells by upregulating the expression of CYLD. (A) The relevant ubiquitin enzymes TTC3, CYLD, TRAF6 and Skp2 were detected by Western blotting. (B) After transfection with sh-CYLD lentivirus, the levels of K63-linked ubiquitination of Akt, phosphorylation of Akt, and CYLD were assayed by Western blotting. (C) After CYLD knockout, the proliferation of HepG2 cell lines in the control, DMSO and BDMC groups was detected by colony formation assay. The values are presented as the means±SD, using one-way analysis of variance (ANOVA) to analyze the differences among groups. *Indicates P<0.05 between groups.