Table 4.
Upstream regulators implicated in the activation of genes in the liver of mice after a 1h-treadmill run
| Transcriptional regulators | Endogenous substances | ||||
|---|---|---|---|---|---|
| Name | Z-score | P-value | Name | Z-score | P-value |
| CREB1* | 4.14 | 1.27E-15 | hydrogen peroxide | 3.438 | 1.74E-09 |
| RELA | 3.40 | 6.55E-09 | Ca2+ | 3.098 | 6.10E-13 |
| FOXO3* | 3.21 | 5.01E-10 | leukotriene D4 | 3.095 | 2.10E-12 |
| HIF1A* | 2.95 | 1.63E-03 | cyclic AMP* | 2.878 | 5.32E-08 |
| FOXO1* | 2.94 | 6.85E-10 | prostaglandin E2 | 2.588 | 1.06E-12 |
| STAT3 | 2.85 | 7.35E-11 | norepinephrine | 2.53 | 4.55E-11 |
| TP63 | 2.75 | 1.79E-04 | corticosterone | 2.414 | 1.13E-05 |
| NFKBIA | 2.72 | 3.32E-08 | fatty acids | 2.387 | 5.18E-06 |
| NFKB1 | 2.60 | 6.96E-06 | histamine | 2.189 | 1.26E-04 |
| SMAD3 | 2.59 | 5.32E-08 | nitric oxide | 2.173 | 3.25E-10 |
| CREM* | 2.50 | 3.83E-10 | |||
| TP53 | 2.49 | 1.33E-06 | |||
| FOXL2 | 2.44 | 2.94E-06 | |||
| CTNNB1 | 2.43 | 2.62E-03 | |||
| MYOD1 | 2.37 | 2.88E-04 | |||
| MEF2C | 2.37 | 2.87E-06 | |||
| NUPR1 | 2.33 | 6.17E-03 | |||
| CDKN2A | 2.32 | 1.38E-08 | |||
| MEF2D | 2.20 | 5.93E-05 | |||
| ELK1 | 2.19 | 1.70E-06 | |||
| EGR1 | 2.18 | 4.98E-06 | |||
| PDX1 | 2.17 | 3.94E-11 | |||
| NFYA | 2.16 | 2.29E-05 | |||
| NRF2* | 2.06 | 3.81E-06 | |||
| STAT6 | 2.00 | 4.19E-05 |
Ingenuity upstream regulator analysis of transcripts that were significantly different (limma t-test P < 0.05 and median fold change > |1.5|) between exercised and sedentary mice immediately after a 1h treadmill run (GSE110747; (19)). Regulators with z-score > 2.0 (ie, predicted to be activated) and with a p-value of overlap <0.05 are shown (other regulator groups are not included). *Transcription regulators discussed as succinate-regulated factors are formatted bold.