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Mouse serum antibody neutralization activity against cholera toxin (CT). Serum samples pooled from each immunization group (n = 10) or the control group mixed with 10 ng CT toxin were added to T-84 cells. Cells were lysed after 3 h incubation, and cell lysates were measured for intracellular cAMP levels by using cAMP EIA kit (Enzo Life Sciences). The mean and standard deviation of each group represented as columns and bars. *** indicates a p-value of <0.001.
