Figure 3.

Mouse serum antibody adherence inhibition activity against ETEC or E. coli bacteria expressing CFA/I, CS1-CS6 adhesins. ETEC or recombinant E. coli expressing CFA/I, CS1, CS2, CS3, CS4/CS6, CS5/CS6, or CS6 adhesin (Table 1), after incubated with mouse serum samples pooled from each immunization group (n = 10) or the control group, were transferred to Caco-2 cells. Incubated for 1 h, cells were washed to remove non-adherent bacteria and lysed. Adherent bacteria were collected, diluted, and plated on LB agar plates. Bacteria were counted for CFUs after overnight growth at 37 ◦C. The number of adherent bacteria in the control group was referred as 100%. ** and *** indicate p-value of <0.01 and <0.001, respectively.