Figure 3.
Voltage-gating properties of mVDAC3 are similar to those of mVDAC1. (A and B) Characteristic bell-shaped plots of normalized average conductance (A) and open probability, Popen (B), as functions of the applied voltage for mVDAC3 and mVDAC1. G/Gmax is the normalized conductance where Gmax is the maximum conductance at voltages closest to 0 mV. Popen is defined as in Eq. 2 (Supplemental methods). Solid lines in B (dark red for mVDAC3; black for mVDAC1) are the fits of Popen plots with the Boltzmann equation (Eq. 3 in Supplemental methods), with gating parameters n and V0 presented in Table 1. Membrane solutions were buffered with 5 mM HEPES at pH 7.4. Membranes were made of PLE with 5% cholesterol (wt/wt). Data are means of 7–11 independent experiments ± SD.