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. 2020 Jan 14;152(2):e201912501. doi: 10.1085/jgp.201912501

Figure 7.

Figure 7.

Tubulin blocks mVDAC3 less efficiently than mVDAC1. (A) Representative current traces for mVDAC3 and mVDAC1 showing time-resolved blockage events recorded at −25 mV applied voltage before and after addition of tubulin at the cis side of the membrane. Current records were digitally filtered at 500 Hz using a low-pass Bessel (8-pole) filter. (B) Voltage dependence of kon of mVDAC3 and mVDAC1 blockage by tubulin. Data are means of 3–10 experiments ± SD. Membrane solutions were buffered with 5 mM HEPES at pH 7.4. PLM were made of PLE with 5% cholesterol (wt/wt).