Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 14;152(2):e201912501. doi: 10.1085/jgp.201912501

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Queralt-Martín et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S3. — Recombinant mouse VDAC3 and VDAC1 isolated from E. coli inclusion bodies (mVDAC3 and mVDAC1) and human VDAC3 and VDAC1 isolated from yeast mitochondria (h_yVDAC3 and h_yVDAC1) form typical VDAC channels. (A and B) Representative single-channel current traces obtained with reconstituted mVDAC3 and mVDAC1 expressed in E. coli and refolded from inclusion bodies (A), and h_yVDAC3 and h_yVDAC1 expressed in yeast and isolated from mitochondria (B), at different applied voltages. Framed traces are displayed in fine time scale on the right. Dashed line indicates zero current level and dotted lines indicate VDAC’s open and closed states. Current records were digitally filtered at 100 Hz using a low-pass Bessel (8-pole) filter. Membrane-bathing solutions consisted of 1 M KCl buffered with 5 mM HEPES at pH 7.4. Planar membranes were formed from soybean PLE with 5% cholesterol (wt/wt).