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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Mol Neurobiol. 2019 Dec 4;57(3):1570–1593. doi: 10.1007/s12035-019-01824-1

Figure 4: Mn2+ increases phosphorylation of IR/IGFR and decreases total protein and mRNA expression.

Figure 4:

A,B) Representative western blot and quantification of p-IGFR expression in STHdh cells following 1hr serum deprivation and 3hr Mn2+ (200/500μM) + IGF(1nM) exposures. Note representative blot is the same samples/run as Figure 1C. Two-way ANOVA; treatment= F(2,6)=5.461; p=<0.0446; genotype= F(1,3)= 30.98; p=0.0114; treatment-genotype interaction= F(2,6)= 3.275; p=0.1093. N=4. C,D) Representative western blot and quantification of pan IGFR protein expression in STHdh cells following 24hr exposure with Mn2+ (50μM) and/or IGF (10nM). Dotted line= Vehicle (=1). Two-way ANOVA for IGFR; treatment= F(3,9)=16.71; p=<0.0005. N=4. E,F) Representative western blot and quantification of pan IR protein expression in STHdh cells following 24hr exposure with Mn2+ (50μM) and/or IGF (10nM). Dotted line= Vehicle (=1). Two-way ANOVA for IR; treatment= F(3,6)=20.59; p=0.0015. N=3. G-I) mRNA expression of IGFR (G), IR (H), and IRS2 (I) after 24hr Mn2+ (50μM) and/or IGF (10nM) exposure. Two-way ANOVA treatment for IGFR: F(3,6)=85.01; p=<0.0001. For IR: F(3,6)=7.204; p=<0.0205. For IRS2: F(1,3)=213.8; p=<0.0007.Error bars= SEM; N=4 for panels A-D, G-I; N=3 for E,F. P<.05, **P<.01, ***P<.001. *= significant by Tukey’s (B) or Dunnet’s (D, F, G, H, I) multiple comparison tests.