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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Mol Neurobiol. 2019 Dec 4;57(3):1570–1593. doi: 10.1007/s12035-019-01824-1

Figure 7: Mn2+-induced p-AKT, IGFR is reduced in HD hiPSC-derived neuroprogenitors.

Figure 7:

A-C) Western blot quantification of hiPSC-derived neuroprogenitors from three control patients and three HD patients (CAG repeat 58, 66, 70) for p-AKT (A), p-IGFR (B), and p-S6 (C), following treatment 3hr treatment with Mn2+ (200/500μM) or Mn2++IGF (1nM) in growth factor/insulin free media, after 3hr serum deprivation. N=8–12 for control from three separate patients, N=7–9 for HD including three separate patients; Error bars= SEM. All data normalized to respective control or HD treated IGF-1 alone. Representative blot in Supplemental Fig 5A,B. One-way ANOVA stats listed in Supp Fig 4D. *= significance from vehicle by Dunnet’s multiple comparison test. *P<.05, **P<.01, ***P<.001.