Skip to main content
. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Forensic Sci Int Genet. 2019 Oct 22;44:102160. doi: 10.1016/j.fsigen.2019.102160

Table 1. A contested hybrid bobcat DNA was positive for feline CCR5 (feCCR5), but negative for enFeLV-LTR, indicating the animal was not a domestic cat hybrid.

PCR results indicate Ct values in parentheses.

Samples/Controls feCCR5 (Ct) enFeLV-LTR (Ct) Comment
Dog - - Negative control for feCCR5 and enFeLV-LTR
Lynx rufus + (25.9 – 27.2) - Samples from verified L. rufus from biorepository (Lee, Malmberg et al. 2017). Positive control for feCCR5, negative control for enFeLV-LTR
Bengal cat + (25.5) + (20.7) Domestic cat-leopard cat hybrid acting as a positive for feCCR5 and enFeLV-LTR (Powers, Chiu et al. 2018).
Domestic cat + (26.4) + (22.0) Positive control for feCCR5 and enFeLV-LTR
‘hybrid’ bobcat -knot wash + (35.1) - Consistent with feline DNA but domestic cat enFeLV-LTR undetected
‘hybrid’ bobcat -rope wash + (32.9) - Consistent with feline DNA but domestic cat enFeLV-LTR undetected