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. 2020 Mar 3;11:246. doi: 10.3389/fmicb.2020.00246

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Copyright © 2020 Talman, Ouologuem, Love, Howick, Mulamba, Haidara, Dara, Sylla, Sacko, Coulibaly, Dao, Sangare, Djimde and Lawniczak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The GQA, a multiplex assay capable of sensitively detecting gametocyte uptake by mosquitoes. (A) RNAseq (López-Barragán et al., 2011) and qPCR fold change of 107 transcripts in gametocytes compared to ring stage parasites. The color scale indicates sex-bias in expression based on (Lasonder et al., 2016) with high female:male ratios in red and balanced ratios in blue. (B) Relative abundance of MDV-1 in blood meals of mosquitoes fed on a serial dilution of gametocytes compared to the maximum gametocyte input (100,000/bloodmeal). Error bars represent the standard deviation. (C) Relationship between observed gametocyte numbers by microscopy and delta Ct of test and control gene in 10 μl blood samples from symptomatic malaria patients colored by asexual parasitemia (26 samples from 6 patients).