Figure 2.

Phenotypic analysis of TEPC and mTEC progenitor subsets in female and male mice during aging. (A) Representative contour plots depicting proportional changes in cTEC^lo subsets in relation to gender with aging. Antibodies against α6-integrin and Sca-1 were used to segregate cTEC^lo into TEPC (α6^hi Sca-1^hi), Sca-1^int cTEC^lo, and Sca-1^lo cTEC^lo subpopulations. (B) Proportion of TEPC, Sca-1^int cTEC^lo, and Sca-1^lo cTEC^lo. (C) Proportion of Ki-67⁺ cells within cTEC^lo subsets. (D) Representative contour plots depicting proportional changes in mTEC^lo subsets in relation to gender with aging. Antibodies against α6-integrin and Sca-1 were used to segregate mTEC^lo into α6^hi Sca-1^hi mTEC^lo, Sca-1^int mTEC^lo, and Sca-1^lo mTEC^lo subpopulations. (E) Proportion of α6^hi Sca-1^hi mTEC^lo, Sca-1^int mTEC^lo, and Sca-1^lo mTEC^lo. (F) Proportion of Ki-67⁺ cells within mTEC^lo subsets. Data presented as mean + SEM (n ≥ 3). * vs. 4 wk, ^∧ vs. 7 wk, ⁺ vs. Female (age matched). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way ANOVA with Tukey's multiple comparisons.