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. 2020 Mar 3;11:302. doi: 10.3389/fimmu.2020.00302

Figure 3.

Figure 3

Evaluation of self-renewal and bipotency in female and male TEPC with aging using in vitro 3D co-cultures. (A) Colony forming efficiency (CFE%) of 4-week-, 7-week-, and 8-month-old TEPCs at D7. Each symbol represents one individual well. (B) Proportion of K14− and K14+colonies. (C) Representative immunofluorescent images of D7 colonies from 4-week-old female TEPC co-cultures. Cortical (β5t; red) and medullary regions (K14; green) from K14− and K14+ colonies are shown, together with nuclear staining (DAPI; blue). Scale bar = 10 μm. (D) Relative Fst and Bmp4 expression in cTEC, mTEClo, mTEChi, and non-TEC subsets from 4-week- and 7-week-old female mice; normalized to 4-week-old cTEC. Data presented as mean + SEM (n ≥ 3). * vs. 4 wk. *p < 0.05, **p < 0.01, ****p < 0.0001, ordinary one- or two-way ANOVA with Tukey's multiple comparisons.