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. 2020 Mar 3;11:302. doi: 10.3389/fimmu.2020.00302

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Copyright © 2020 Hun, Wong, Gunawan, Alsharif, Quinn and Chidgey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic analysis of Aire⁺ mTECs and cTECs in middle-aged female and male mice with degarelix treatment following Cy damage. (A) Representative contour plots depicting proportional changes in the Aire⁺ mTEC subpopulation with or without degarelix post-Cy treatment. (B) Proportion and number of Aire⁺ mTECs per thymus. (C) Representative contour plots depicting proportional changes in the Aire⁺ cTEC subpopulation with or without degarelix post-Cy treatment. (D) Proportion and number of Aire⁺ cTECs per thymus. Data presented as mean + SEM (n ≥ 3). * vs. Cy, ⁺ vs. UT. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way ANOVA with Sidak's multiple comparisons.