Figure 8.

Phenotypic analysis of mTEC progenitor subsets in middle-aged female and male mice with degarelix treatment following Cy damage. (A) Representative contour plots depicting proportional changes in mTEC^lo subsets with or without degarelix post-Cy treatment. Antibodies against α6-integrin and Sca-1 were used to segregate mTEC^lo into α6^hi Sca-1^himTEC^lo, Sca-1^int mTEC^lo, and Sca-1^lo mTEC^lo subpopulations. (B) Proportion of α6^hi Sca-1^hi mTEC^lo, Sca-1^int mTEC^lo, and Sca-1^lo mTEC^lo. (C) Proportion of Ki-67⁺ cells within mTEC^lo subsets. Data presented as mean + SEM (n ≥ 3). * vs. Cy, ⁺ vs. UT. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary two-way ANOVA with Sidak's multiple comparisons.