Table 1.

Antibodies utilized for immunofluorescent staining—flow cytometric analysis.

Antibody	Marker	Host/isotype/clone	Supplier
CD45	Thymocyte	Rat IgG2b, κ 30-F11	BD Biosciences, U.S.A.
TER119	Erythrocyte	Rat IgG2b, κ TER-119	BD Biosciences, U.S.A.
EpCAM	Epithelial	Rat IgG2a, κ G8.8	Biolegend, U.S.A.
UEA-1	Medullary TEC	Lectin	Vector Labs, U.S.A.
Ly-51	Cortical TEC	Rat IgG2a, κ 6C3	BD Biosciences, U.S.A.
MHCII	TEC maturity	Rat IgG2b, κ M5/114.15.2	Biolegend, U.S.A.
α6-integrin	TEPC	Rat IgG2a, κ GoH3	Biolegend, U.S.A.
Sca-1	TEPC	Rat IgG2a, κ D7	eBioscience, U.S.A.
Ki-67	Proliferation	Mouse IgG1, κ B56	BD Biosciences, U.S.A.
Aire	Autoimmune regulator	Rat IgG2c, κ 5H12	Invitrogen, U.S.A.
Streptavidin	Biotin	Protein	BD Biosciences, U.S.A.
CD44	Activated T-cell	Rat IgG2b, κ IM7	eBioscience, U.S.A.
CD25		Rat OFA IgG1, λ PC61	BD Biosciences, U.S.A.
CD117		Rat IgG2b, κ 2B8	eBioscience, U.S.A.
CD3ε	Thymocyte	Ar hamster IgG1, κ 145-2C11	BD Biosciences, U.S.A.
CD4	Helper T-cell	Rat IgG2b, κ RM4-5	BD Biosciences, U.S.A.
CD8	cytotoxic T-cell	Rat LOU IgG2a, κ 53-6.7	BD Biosciences, U.S.A.
CD19		Rat LEW IgG2a, κ 1D3	BD Biosciences, U.S.A.
CD11b		Rat IgG2b, κ M1/70	BD Biosciences, U.S.A.
CD11c		Ar Hamster IgG1, λ2 HL3	BD Biosciences, U.S.A.
Gr-1/Ly-6G		Rat IgG2b, κ RB6-8C5	BD Biosciences, U.S.A.
CD45R/B220	B-cell	Rat IgG2a, κ RA3-6B2	BD Biosciences, U.S.A.
NK1.1		Mouse IgG2a, κ PK136	BD Biosciences, U.S.A.
H-2kb		Mouse BALB/c IgG2a, κ AF6-88.5	BD Biosciences, U.S.A.
CD62L	Naïve T-cell	Rat IgG2a, κ MEL-14	eBioscience, U.S.A.
Foxp3		Rat IgG2a, κ FJK-16s	eBioscience, U.S.A.
TCRβ	T-cell	Ar Hamster IgG2, λ1 H57-597	BD Biosciences, U.S.A.
CD49d		Rat IgG2b, κ R1-2	Biolegend, U.S.A.
CD122		Rat SD IgG2b, κ TM-β1	BD Biosciences, U.S.A.