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. 2020 Mar 9;11:1282. doi: 10.1038/s41467-020-15042-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Time-course IF analysis (representative of two hPGCLC inductions) showing PRDM14 expression in embryoid body (EB) sections throughout hPGCLC specification from PRDM14-AID-Venus fusion reporter cell line. hPGCLCs were induced by cytokines and EBs were collected at 12 h, and on D1-D5. Representative images for 12 h, D1, D2 and D4 are shown. hPGCLCs were marked by SOX17 and highlighted by a dashed line. Arrows show examples of SOX17⁺ cells that are PRDM14-negative at 12 h and PRDM14-positive on D1 of differentiation. Scale bar is 60 μm. b Quantification of results from a and Supplementary Fig. 2A, including the D1–D5 time points. Data show the percentage of PRDM14-Venus⁺SOX17⁺ cells as mean ± SD of n = 11 EB sections from two hPGCLC inductions. c Quantification of results from (Supplementary Fig. 2A) showing the percentage of PRDM14-Venus⁺SOX17⁺OCT4⁺ cells as mean ± SD of n = 3 EB sections from one hPGCLC induction.