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. 2020 Mar 9;11:1282. doi: 10.1038/s41467-020-15042-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Experimental design and representative flow cytometry plots showing reduced hPGCLC induction in hormone-sensitive clones, but not in the parental control. hPGCLCs were defined as NANOS3-tdTomato⁺AP⁺ cells. b Quantification of flow cytometry results for PRDM14-AID-Venus hPGCLCs induced with or without IAA (100 μM). The induction efficiency of untreated cells (“no IAA”) was set to 1. Data show mean ± SD for n = 5 for cl11 and “no TIR1” control and n = 6 for cl21, ns not significant (P = 0.1308), ****P < 0.0001 (Two-way ANOVA followed by Sidak’s multiple comparison test). c Quantification of flow cytometry results for PRDM14-JAZ-Venus hPGCLCs. The induction efficiency of untreated cells (“no Cor”) was set to 1. Data show mean ± SD for n = 4 independent experiments per clone, ns not significant (P = 0.9979), ****P < 0.0001 (Two-way ANOVA followed by Sidak’s multiple comparison test). d Time-course of PRDM14 depletion. IAA (500 μM) was added on indicated days of hPGCLC induction from PRDM14-AID-Venus clones. Note that IAA was not washed off until the end of differentiation (D4), when hPGCLC induction efficiency was assessed by flow cytometry. Data show mean ± SD of n = 3 for PRDM14-AID or mean of n = 2 for “no TIR1” control. Statistical significance was analysed by two-way ANOVA followed by Sidak’s multiple comparison test. For PRDM14-AID: no IAA vs. D0 ***(P = 0.0003), no IAA vs. D1 ***(P = 0.0008), no IAA vs. D2 **(P = 0.0018), no IAA vs. D3 ns (not significant, P = 0.9614); for “no TIR1”: all ns not significant (P > 0.99).