Table 3.
Buffer recipes for ChIP.
| Buffer A | 1x |
| Buffer A stock, µl | 250 |
| H2O, µl | 750 |
| DTT, µl | 0.5 |
| PIC, µl | 5 |
| Buffer B | 1x |
| Buffer B stock, µl | 275 |
| H2O, µl | 825 |
| DTT, µl | 0.55 |
| ChIP buffer 1 | 1x |
| ChIP buffer stock, µl | 10 |
| H2O, µl | 90 |
| PIC, µl | 0.5 |
| ChIP buffer 2 | 1x |
| ChIP buffer stock, µl | 40 |
| H2O, µl | 360 |
| PIC, µl | 2 |
| tRNA (Sigma) [10 mg/ml], µl | 2 |
| Elution | 1x |
| Elute buffer, µl | 25 |
| H2O, µl | 25 |
| Blocking buffer | 1x |
| ChIP buffer (CST), µl | 150 |
| H2O, µl | 1110 |
| PIC (Roche), µl | 75 |
| BSA (Sigma) (10%), µl | 150 |
| tRNA (Sigma) [10 mg/ml], µl | 15 |
| Low salt | 1x |
| ChIP buffer (CST), µl | 300 |
| H2O, µl | 2700 |
| High salt | 1x |
| ChIP buffer (CST), µl | 100 |
| H2O, µl | 900 |
| NaCl, µl | 70 |
| LiCl Wash buffer | For 250 mL |
| 20 mM Tris-HCl pH 8.0, ml | 5 ml of 1M |
| 2 mM EDTA, ml | 1 ml of 0.5 M |
| 250 mM LiCl, g | 2.65 g |
| 1% NP-40, ml | 2.5 ml |
| 1% Deoxycholate, g | 2.5 g |
| H2O, ml | 241.5 ml |