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. 2020 Feb 20;52(2):213–226. doi: 10.1038/s12276-020-0383-3

Fig. 2. hCdH generation and in vivo transplantation of hCdHs into liver disease model mice.

Fig. 2

a Freshly isolated human primary hepatocytes were cultured for 7 days in HAC-containing reprogramming medium. The morphology of human primary hepatocytes (hPHs) changes rapidly in the reprogramming medium, and the capacity to proliferate is acquired. Scale bars, 100 μm. b After generation, human chemically derived hepatic progenitors (hCdHs) effectively express the progenitor markers alpha fetoprotein (AFP) and SOX9. Scale bars, 50 μm. c A liver paraffin Section 3 weeks after mCherry-tagged hCdHs were transplanted into Alb-TRECK/SCID mice. Liver sections were stained with human albumin (ALB, green, left), human cytokeratin 7 (CK7, green, right), and mCherry (red, both), and the nuclei were counterstained with Hoechst. Scale bars, 50 μm.