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. 2020 Mar 9;11:1269. doi: 10.1038/s41467-020-15002-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Model depicting zebrafish embryo (top) and KV morphology (bottom) during development. Approximate location of KV denoted by magenta spot. KV membrane (magenta) and regions of apical polarity (black) depicted in model below. b Top: maximum confocal projections of KV at developmental stages denoted in a. pH3 (mitotic nuclei, cyan) and KV membrane marker (Sox17:GFP-CAAX, magenta) shown. Bottom: KV membrane marker (Sox17:GFP-CAAX—gray) and lumen trace (orange) shown. Bars, 50 μm. c Mitotic indices (%) represented as violin plot with endpoints depicting minimum and maximum values, quartiles depicted by thin black lines, median depicted by thick black line. n > 247 cells/stage, n = 43 embryos, two-tailed, unpaired Student’s t-test. Statistical results detailed in Supplementary Table 5. d Representative 3D renderings of KV under conditions of DMSO vehicle control, microtubule inhibition (1 μM nocodazole), or PLK1 inhibition (1 μM BI2536). Sox17:GFP-CAAX (magenta), pH3-positive nuclei (cyan), and DAPI (blue) shown on the left. Sox17:GFP-CAAX (gray) and lumen trace (orange) shown on the right. Percentages indicate mitotic index of image, lumen area denoted. Bar, 20 μm. e Violin plot depicting normalized 2D lumen area under conditions represented in d with endpoints depicting minimum and maximum values, quartiles depicted by thin black lines, and median depicted by thick black line. One-way ANOVA with Dunnett’s multiple comparison and statistical results are detailed in Supplementary Table 5 (****p < 0.0001 for n > 41 embryos).