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. 2020 Mar 9;10:4291. doi: 10.1038/s41598-020-61042-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time course analysis of OY phytoplasma accumulation in M. striifrons by qPCR. Accumulations of OY phytoplasmas in the entire insect body (A) and in the alimentary canal, hemocoel, and salivary glands (B) were quantified at different days after acquisition start by qPCR. Only OY phytoplasma-positive samples were used to measure the relative phytoplasma titer. Each red point represents an OY-positive sample. Four dissected organs/tissues were pooled as one sample. Thick horizontal line in each boxplot represents the median of each dataset. Statistical significance was determined using Tukey’s honestly significant difference test. Only significant comparisons are shown. *p < 0.05, **p < 0.01.