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. 2020 Mar 9;11:1283. doi: 10.1038/s41467-020-14932-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Bleaching curves of individual mCherry-M2x24 foci in fixed HEK293T cells using different media (PKM buffer—yellow, Vectashield® - blue and Prolong® Diamond – black) upon 5 s intervals of constant illumination. b Widefield and SIM maximum projections of the β-actin-∆3′UTR-M2x24 construct (green) costained with Phalloidin-AF647 (red) and Hoechst 33258 (blue), scale bar = 10 µm. HEK293T cells. c Mean intensity bleaching curves of Hoechst, Mango and Phalloidin signal during structured illumination acquisition of β-actin-∆3’UTR-M2x24 expressing HEK293T cells. The standard deviation is plotted as the shaded area around each curve obtained from eight different fields of view. d Widefield and SIM maximum projections of NEAT-1 v1-M2x24 construct (green) costained NONO (magenta) and Hoechst 33258 (blue) in HEK293T cells. Zoom of highlighted foci shown as inset. Large scale bars = 5 µm, inset scale bars = 0.5 µm. Inset intensity profile (lower right) of annotated NONO foci (magenta) from SIM maximum projection showing the peripheral NEAT-1 signal (green).