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. 2020 Mar 9;10:4326. doi: 10.1038/s41598-020-61405-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Human iPSCs and basic characterization of iPSC-derived mesenchymal stem cells (iPSC-MSCs). (A) Morphology and alkaline phosphatase (AP) activity of iPSC colony. (B) Immunostaining of human iPSCs for the pluripotent markers Oct4, Nanog, TRA-1-81 and SSEA4. (C) Morphological changes during MSC differentiation via embryonic body cultivation. (D) MSC surface marker characterization by FACS analysis. (E) Multilineage differentiation of iPSC-derived MSCs. Oil red O, von Kossa, and Myo D (immunostaining) were used to assess the multilineage differentiation of iPSC-derived MSCs for adipogenesis, osteogenesis and myogenesis, respectively.