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. 2020 Mar 10;6:12. doi: 10.1038/s41421-020-0144-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Illustration of the m¹A-modified RNA probe with stem and loop structure derived from the known m¹A 1322 site in human 28S rRNA. Red asterisks indicated the m¹A-modified sites. b Dot-blot analysis of the m¹A probes specificity with m¹A or m⁶A antibody. c IP and immunoblot analyses of the indicated probes in trophoblast HTR8/SVneo transfected with YTHDF1/2/3 plasmid as indicated. Data are representative of three independent experiments.