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. 2020 Mar 9;10:4307. doi: 10.1038/s41598-020-61275-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of promoter selectivity for tumour cell lines. The activity of the selected gene promoters was assessed by SEAP reporter assays. (a) The activity of Survivin/BIRC5, CXCR4 and TERT promoters was assessed in tumour cells (SAN, A375, SKOV3, HEK293) and in normal lung fibroblasts (MRC5). The chart reports the relative strength of each promoter in the indicated cells, normalized to the activity of the housekeeping CMV enhancer/promoter construct. Statistical significance compares the promoter strength in normal MRC5 to corresponding tumour cell line. The asterisks indicate the P values: ***P < 0.0005; ****P < 0.00005 in the comparisons to the reporter activities shown by MRC5 cells. (b) SKOV3 cells were enriched in the G2/M phase of the cell cycle with 0.1 µg/ml of nocodazole in DMSO for 12 hours; Survivin/BIRC5 promoter activity of G2/M synchronized cells was compared to unsynchronized, DMSO-treated SKOV3 cultures. The asterisks indicate the P value: ***P < 0.0005.