Figure 3.

Evaluation of viral replication and cytopathic effect of Survivin_oHSV in tumour and normal cell lines. (a) Replicative potential of the wild-type R-LM55 and of Survivin_oHSV were evaluated in the survivin positive tumour cell lines (SKOV3, SAN and A375), and in normal MRC5 cells. Cells were infected at MOI 0.1 (PFU/cell), and the genome copies were determined 24 h post infection. All the differences in viral genome copies replication are statistically significant (≤0.01). (b) The panels show the viral replication 24 h post infection at 0.1 multiplicity of infection with the RC Survivin_oHSV and wt R-LM55 viruses. The viral spread is evaluable as eGFP positive cells under fluorescence microscopy; the cytopathic effect is evident as round and dethatched cells by phase-contrast microscopy.