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. 2020 Mar 9;10:4307. doi: 10.1038/s41598-020-61275-w

Table 1.

Oligonucleotide sequences.

Step I RC1_Fwd 5′-gcccggggacggccaacgggcgcgcggggctcgtatctcattaccgccgaacccctatttgtttatttttct-3′
Step I RC1_Rev 5′-gcggtcccgcgtcgggtcgtggatccgtgtcggcagccgcgctccgtgtgttatttgttaactgttaattgtc-3′
Step II RC1_Fwd 5′-gcccggggacggccaacgggcgcgcggggctcgtatctcattaccgccgagttctttgaaagcagtcgag-3′
Step II RC1v1_Fwd 5′-cccggggacggccaacgggcgcgcggggctcgtatctcattaccgccgaatatggatcctatggcgcggttctttgaaagcagtcgag-3′
Step II RC1_Rev 5′-gcggtcccgcgtcgggtcgtggatccgtgtcggcagccgcgctccgtgtggccgccgccgccacctct-3′
Step I RC2_Fwd 5′-gggaagtcggggcccgggccccgcccccggcccgttcctcgttagcatgcacccctatttgtttatttttct-3′
Step I RC2_Rev 5′-gccggggcgctgcttgttctccgacgccatcgccgatgcggggcgatcctttatttgttaactgttaattgtc-3′
Step II RC2_Fwd 5′-gggaagtcggggcccgggccccgcccccggcccgttcctcgttagcatgcgttctttgaaagcagtcgag-3′
Step II RC2v1_Fwd 5′-gggaagtcggggcccgggccccgcccccggcccgttcctcgttagcatgcatatggatcctatggcgcggttctttgaaagcagtcgag-3′
Step II RC2_Rev 5′-gccggggcgctgcttgttctccgacgccatcgccgatgcggggcgatcctgccgccgccgccacctct-3′
Step I gD_Fwd 5′-ttgtcgtcatagtgggcctccatggggtccgcggcaaatatgccttggcgacccctatttgtttatttttct-3′
Step I gD _Rev 5′-atcgggaggctggggggctggaacgggtccggtaggcccgcctggatgtgttatttgttaactgttaattgtc-3′
Step II gD _Fwd 5′-ttgtcgtcatagtgggcctccatggggtccgcggcaaatatgccttggcggagaattccgatatccagatgacccagtccc-3′
Step II gD _Rev 5′-atcgggaggctggggggctggaacgggtccggtaggcccgcctggatgtgggatccaccggaaccagagc-3′
Taqman DNApolFw 5′-catcaccgacccggagagggac-3′
Taqan DNApolRev 5′-gggccaggcgcttgttggtgta-3′
Taqman Probe FAM-ccgccgaactgagcagacacccgcgc-Tamra