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. 2020 Feb 3;52(2):238–252. doi: 10.1038/s12276-020-0379-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative images showing interscapular brown adipose tissue (iBAT) deposits (n = 6 per group). b Hematoxylin and eosin (H&E) staining of the iBAT section (5 μm) from the WT or IDH2KO mice exposed to a high-fat diet for 4 weeks. c PET/CT images showing the ¹⁸F-FDG uptake in the iBAT of the WT and IDH2KO groups as described in the “Materials and methods” (n = 5 per group). d Quantitative positron emission tomography–computed tomography (PET/CT) region of interest (ROI) analysis of the ¹⁸F-FDG uptake in the iBAT; ¹⁸F-FDG: 2-deoxy-2-[¹⁸F]-fluoro-d-glucose.