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. 2020 Mar 9;10:4276. doi: 10.1038/s41598-020-61116-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative genome hybridization with FITC-labelled probe specific for male (green) and rhodamine-labelled probe specific for female (red) genomic content in Chelodina expansa (a–d), Chelodina novaeguineae (e–h), and Elseya novaeguineae (i–l). Chromosomal regions with similar genomic content between sexes are visualized in yellow. The white arrow indicates male-specific region (green), corresponding to the Y chromosome.