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. 2020 Mar 3;7:37. doi: 10.3389/fmolb.2020.00037

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Copyright © 2020 Jiao, Zhao, Wang, Ren and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MCC950 inhibits NLRP3 inflammasome activity and pro-inflammatory cytokine release. (A) We conducted a CoIP analysis to assess MCC950 (50 μM)-induced inhibition of NLPR3 inflammasome activation in OGD- or LPS-exposed spinal neurons. Left: WB analysis of NLRP3, ASC, and Caspase-1 (p20) levels in lysates/input. Right: We conducted CoIP analysis using an anti-NLRP3 antibody and subjected the CoIP products to WB analysis of NLRP3, ASC, and pro-Caspase-1 levels. (B) The levels of TNF-α, IL-1β, and IL-18 release. Data were reported as the mean ± SEM, n = 5. One-way ANOVA, *p < 0.05, ***p < 0.001, vs. control; ^#p < 0.05, ^###p < 0.001, vs. OGD; ^&p < 0.05, ^&&&p < 0.001, vs. LPS. OGD, oxygen–glucose deprivation; LPS, lipopolysaccharides; CoIP, co-immunoprecipitation; WB, Western blot; SEM, standard error of the mean.