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. 2020 Feb 20;52(2):281–292. doi: 10.1038/s12276-020-0389-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Differential scanning fluorimetry assays. The right panel shows the first derivative of the thermal denaturation profiles of fascin1 in the absence (black) and in the presence of 1 mM imipramine (red in triplicate). The left panel summarizes the changes in T_m induced by the presence of the selected compounds at 1 mM in 10% DMSO. b Fluorescence titration. The right panel shows the fluorescence emission spectra of fascin1 in the presence of increasing concentrations of imipramine. The left panel shows the binding isotherm. White circles represent the center of mass of the different spectra, and the continuous lines symbolize the best fit of the data to a one-site binding model.