Fig. 5.

The effect of combined treatment with necrostatin-1 (Nec-1) and caspase-3 inhibitor (Ac-DEVD-CHO) (a, b) or Nec-1 and curcumin (Curc) against the hydrogen peroxide-induced cell damage in UN- (a, c) and RA- (b, d) SH-SY5Y cells. The cells were pre-treated for 30 min with Nec-1 (20 μM) and Ac-DEVD-CHO (20 μM) or Nec-1 (20 μM) and Curc (5 μM) alone or in combination followed by 24 h of treatment with H₂O₂ (0.25 mM and 0.5 mM for UN- and RA-SH-SY5Y cells). Cell viability was estimated by the MTT reduction assay, and data were normalized to vehicle-treated cells (control) and are presented as the mean ± SEM from 3 separate experiments with 5 repetitions each. *P < 0.05 and ***P < 0.001 vs. vehicle-treated cells; ^#P < 0.05 and ^###P < 0.001 vs. H₂O₂-treated cells; ^&P < 0.05 and ^&&&P < 0.001 vs. H₂O₂ + Nec-1 + Curc-treated cells