Fig. 8.

a, b The effect of necrostatin-1 (Nec-1) on the H₂O₂-induced 145 kDa and 120 kDa spectrin α II breakdown products in UN- and RA-SH-SY5Y cells, which are specifically cleaved by calpains and caspases, respectively. Cells were pre-treated for 30 min with Nec-1 (20 μM) or calpain inhibitor MDL28170 (10 μM), followed by 14 h of treatment with H₂O₂ (0.25 and 0.5 mM for UN- and RA-SH-SY5Y cells, respectively). c The effect of Nec-1 on the H₂O₂-induced increase in cytosolic AIF (apoptosis inducing factor) level. The UN-SH-SY5Y cells were pre-treated for 30 min with Nec-1 (20 μM) followed by 14 h of treatment with H₂O₂ (0.25 mM). a–c Histograms show the quantified Western blot results from duplicate determinations in 2–3 independent experiments which were normalized to the protein loading control (GAPDH) and are expressed as fold of the control ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. vehicle-treated cells; ^#P < 0.05 vs. H₂O₂-treated cells