Table 1.

The effect of necrostatin-1 on 6-OHDA-induced cell damage in UN- and RA-SH-SY5Y cells

	UN-SH-SY5Y	RA-SH-SY5Y
Control	99.9 ± 0.1	100.0 ± 0.0
6-OHDA	62.8 ± 0.6***	68.6 ± 0.2***
+ Nec-1 20	72.0 ± 2.6***,#	70.2 ± 2.0***
+ Nec-1 40	78.2 ± 5.6***, ##	81.2 ± 3.8**, #
+ NAC	81.4 ± 4.7***, ###	83.2 ± 4.0**, #

UN- and RA-SH-SY5Y cells were pre-treated for 30 min with necrostatin-1 (Nec-1; 20 and 40 μM) followed by 24 h of treatment with 6-hydroxydopamine (6-OHDA, 0.1 and 0.2 mM for UN- and RA-SH-SY5Y cells, respectively)

An antioxidant N-acetylcysteine (NAC, 1 mM) was given to cells concomitantly with 6-OHDA. The MTT reduction assay was employed for cell viability assessment

Data were normalized to the vehicle-treated cells and are presented as the mean ± SEM from 4 separate experiments with 5 repetitions each

**P < 0.01 and ***P < 0.001 vs. vehicle-treated cells

^#P < 0.05, ^##P < 0.01, and ^##P < 0.001 vs. 6-OHDA-treated cells