Table 2.
The effect of necrostatin-1 on hydrogen peroxide-induced caspase-3 activity in UN- and RA-SH-SY5Y cells
| UN-SH-SY5Y | RA-SH-SY5Y | RA-SH-SY5Y | |
|---|---|---|---|
| 9 h | 9 h | 18 h | |
| Control | 100.0 ± 8.0 | 100.0 ± 2.7 | 100.0 ± 5.8 |
| Nec-1 20 | 95.9 ± 0.3 | 109.3 ± 5.2 | 119.8 ± 5.9 |
| H2O2 | 753.8 ± 20.5 *** | 229.6 ± 18.9 *** | 487.9 ± 44.9 *** |
| + Nec-1 1 | 854.7 ± 31.5 *** | 236.9 ± 5.8 ** | 668.0 ± 64.3 *** |
| + Nec-1 10 | 746.1 ± 39.2 *** | 252.9 ± 12.2 *** | 568.1 ± 52.8 *** |
| + Nec-1 20 | 760.9 ± 19.8 *** | 271.9 ± 8.2 *** | 520.1 ± 27.5 *** |
| + Ac-DEVD-CHO | 33.5 ± 1.1 ### | 24.0 ± 14.0 ### | 19.9 ± 8.9 ### |
UN- and RA-SH-SY5Y cells were pre-treated for 30 min with necrostatin-1 (Nec-1; 1–20 μM) followed by 9 or 18 h of treatment with H2O2 (0.25 and 0.5 mM for UN- and RA-SH-SY5Y, respectively)
As a positive control for the assay we used Ac-DEVD-CHO (20 μM), an inhibitor of caspase-3 which was given 30 min before the cell damaging factor
Data were normalized to vehicle-treated cells (control) and are presented as the mean ± SEM from 3 separate experiments with 2 repetitions each
**P<0.01 and ***P < 0.001 vs. vehicle-treated cells
###P < 0.001 vs. H2O2-treated cells