Table 4.

The effect of necrostatin-1 and pepstatin A alone and in combination against the hydrogen peroxide-induced cell damage in UN-SH-SY5Y cells

	Cell viability	LDH release
	(% control)	(% control)
Control	100.0 ± 9.9	100.2 ± 6.4
H2O2	60.6 ± 4.3 ***	358.3 ± 17.2 ***
+ Nec-1	90.3 ± 4.1 ##	277.2 ± 14.1 ***, ##
+ PsA	88.9 ± 3.7 #	271.8 ± 10.2 ***, ###
+PsA + Nec-1	89.1 ± 3.3 #	280.3 ± 16.2 ***, ##

UN-SH-SY5Y cells were pre-treated for 30 min with necrostatin-1 (Nec-1; 20 μM) and pepstatin A (PsA; 0.2 μM) alone and in combination followed by 24 h of treatment with H₂O₂ (0.25 mM)

The MTT reduction and LDH release assays were employed for cell viability and toxicity assessments, respectively

Data were normalized to the vehicle-treated cells and are presented as the mean ± SEM from 3 separate experiments with 5 repetitions each

***P < 0.001 vs. vehicle-treated cells

^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. H₂O₂-treated cells