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. 2020 Mar 9;10:4308. doi: 10.1038/s41598-020-61246-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-transfection of miR-335 inhibitor or NC together with Keima Red. (A) Confocal images showed an increased pool of mitochondria 48 and 72 h after transfection with the miR-335 inhibitor.0 (B) 561/458 nm excitation ratio showed an increase number of red mitochondria (excited at 561), indicating there localization in an acidic environment. The graphic representation was created using GraphPad Prism, version 5.03, (https://www.graphpad.com/scientific-software/prism/).