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. 2020 Mar 9;10:4287. doi: 10.1038/s41598-020-61257-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Segmentation of the substantia nigra and red nucleus in the second experiment, overlaid on the average EPI image. In blue, tissue classified as substantia nigra by SPM’s segmentation algorithm through its low intensity in the T2*-weighted images used in the segmentation. In orange/yellow, voxels classified as red nucleus. Coordinates in MNI space. (b) Midbrain mask used for the region of interest correction in Experiments 2 and 3. RN: red nucleus; SNhr: hypointense region, corresponding to iron-rich tissue attributed to the substantia nigra pars reticulata; SNc: region between RN and SNhr not classified as hypointense, attributed to substantia nigra pars compacta; CP: cerebellar peduncle; VTA: ventral tegmental area; IPF: interpeduncular fossa; III: third ventricle.