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. 2020 Mar 9;10:4338. doi: 10.1038/s41598-020-61328-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo hair growth enhancement with MB-enhanced single- or dual-frequency US sonication. (A) Gross observations of the dorsal skin of C57BL/6 mice. The dorsal skin surfaces of the mice were shaved, and then test compounds and treatments were topically applied in group C (Control), S (single frequency US), D (dual frequency US), S + MB10 (single frequency US combined with 10-fold dilution of microbubbles), and D + MB10 (dual frequency US combined with 10-fold dilution of microbubbles) for 3 weeks. (B) Quantification of hair growth rates on the dorsal skin after shaving the hair in various mouse groups over 21 days. Data are mean and SD values (n = 5).