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Knockdown of LCAL62 decreases SMAD2/3 binding at the FOXA2 promoter. (a) ChIP-qPCR shows SMAD2/3 occupancy at two genomic locations of the FOXA2 promoter in wild type and LCAL62-shRNA A549 cells. IgG was used as a negative control. Primer locations are depicted in the schematic above. n of 2 (b) qRT-PCR confirmation of expression of LCAL62 and FOXA2 in cell lines.
