Table 5.

Liposome surface engineered with different enzymes to study different biochemical activities

S. no	Enzymosome	Liposome application	References
1	Brain glutamate-decarboxylase	To study the comparison of pyridoxal phosphate (PLP) activation and inhibition kinetics by PLP oxime-o-acetic acid between free and liposome bound form	Covarrubias and Tapia (1980)
2	SOD	To study retention, efficiency, enzymatic activity, incorporation, protein–lipid ratio and zeta potential between conventional and long circulating liposome (LCL)	Gaspar et al. (2003)
3	SOD	SOD-liposome and SOD-enzymosome were used to compare their therapeutic activities on different animal models for oxidative stress and liver ischemia,	Corvo et al. (2015)
4	Catalase	To study controlled oxidation of glucose by encapsulated glucose oxidase with decomposition of H2O2	Yoshimoto et al. (2010)
5	Catalase–sSOD	Stability studies including half-life and activity	Skólmowska and Kmieć (2011)
6	C. utilis Uricase	Comparison between in vitro uricolytic activity of free and PEGylated uricase and in vivo uricase studies of its enzymosome in animal models	Tan et al. (2012)
7	NTP Dase-1	To study the inhibition of platelet activation induced by ADP, collagen or thrombin	Chaikof et al. (2006)
8	β-glucuronidase	Immunoliposome directed against human ovarian cancer cells to study anti-tumor activity	Fonseca et al. (1999)
9	Carboxypeptidase	To study membrane targeting and biological functions	Mima et al. (2006)
10	Carbonic anhydrase	to study protein membrane interaction and refolding of denatured enzyme	Yoshimoto et al. (1998)
11	Glucose oxidase	To study the sensitivity of liposomes for glucose concentration	Jo et al. (2008)